lectin conjugates Search Results


asgr1 antibody  (Proteintech)
93
Proteintech asgr1 antibody
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Asgr1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/asgr1 antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
asgr1 antibody - by Bioz Stars, 2026-02
93/100 stars
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anti mac 2  (Proteintech)
93
Proteintech anti mac 2
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Anti Mac 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mac 2/product/Proteintech
Average 93 stars, based on 1 article reviews
anti mac 2 - by Bioz Stars, 2026-02
93/100 stars
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conjugated lectin lcafitc  (Cosmo Bio USA)
90
Cosmo Bio USA conjugated lectin lcafitc
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Conjugated Lectin Lcafitc, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/conjugated lectin lcafitc/product/Cosmo Bio USA
Average 90 stars, based on 1 article reviews
conjugated lectin lcafitc - by Bioz Stars, 2026-02
90/100 stars
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fluorescein isothiocyanate-conjugated lectin from arachis hypogaea (peanut) (peanut agglulutinin: pna)  (Merck KGaA)
90
Merck KGaA fluorescein isothiocyanate-conjugated lectin from arachis hypogaea (peanut) (peanut agglulutinin: pna)
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Fluorescein Isothiocyanate Conjugated Lectin From Arachis Hypogaea (Peanut) (Peanut Agglulutinin: Pna), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein isothiocyanate-conjugated lectin from arachis hypogaea (peanut) (peanut agglulutinin: pna)/product/Merck KGaA
Average 90 stars, based on 1 article reviews
fluorescein isothiocyanate-conjugated lectin from arachis hypogaea (peanut) (peanut agglulutinin: pna) - by Bioz Stars, 2026-02
90/100 stars
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lectin-fitc conjugate l4895  (Merck KGaA)
90
Merck KGaA lectin-fitc conjugate l4895
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Lectin Fitc Conjugate L4895, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lectin-fitc conjugate l4895/product/Merck KGaA
Average 90 stars, based on 1 article reviews
lectin-fitc conjugate l4895 - by Bioz Stars, 2026-02
90/100 stars
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fluorescence-conjugated lycopersicon esculentum lectin  (BioActs Inc)
90
BioActs Inc fluorescence-conjugated lycopersicon esculentum lectin
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Fluorescence Conjugated Lycopersicon Esculentum Lectin, supplied by BioActs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence-conjugated lycopersicon esculentum lectin/product/BioActs Inc
Average 90 stars, based on 1 article reviews
fluorescence-conjugated lycopersicon esculentum lectin - by Bioz Stars, 2026-02
90/100 stars
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griffonia simplicifolia lectin-i (gs-i) conjugated dylight 488  (EY Laboratories)
90
EY Laboratories griffonia simplicifolia lectin-i (gs-i) conjugated dylight 488
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Griffonia Simplicifolia Lectin I (Gs I) Conjugated Dylight 488, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/griffonia simplicifolia lectin-i (gs-i) conjugated dylight 488/product/EY Laboratories
Average 90 stars, based on 1 article reviews
griffonia simplicifolia lectin-i (gs-i) conjugated dylight 488 - by Bioz Stars, 2026-02
90/100 stars
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wheat germ agglutinin biotin conjugated triticum vulgare lectin  (EY Laboratories)
90
EY Laboratories wheat germ agglutinin biotin conjugated triticum vulgare lectin
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Wheat Germ Agglutinin Biotin Conjugated Triticum Vulgare Lectin, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wheat germ agglutinin biotin conjugated triticum vulgare lectin/product/EY Laboratories
Average 90 stars, based on 1 article reviews
wheat germ agglutinin biotin conjugated triticum vulgare lectin - by Bioz Stars, 2026-02
90/100 stars
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biotin-conjugated lycopersicon esculentum lectins  (EY Laboratories)
90
EY Laboratories biotin-conjugated lycopersicon esculentum lectins
( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. <t>ASGR1</t> was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.
Biotin Conjugated Lycopersicon Esculentum Lectins, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin-conjugated lycopersicon esculentum lectins/product/EY Laboratories
Average 90 stars, based on 1 article reviews
biotin-conjugated lycopersicon esculentum lectins - by Bioz Stars, 2026-02
90/100 stars
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lectin sna-alkaline phosphatase conjugate la-6802-1  (EY Laboratories)
90
EY Laboratories lectin sna-alkaline phosphatase conjugate la-6802-1
( a ) Western blot analysis showing the absence/presence of Neu5Gc in serum samples (10 μg) when tested with anti-Neu5Gc antibody. ( b ) Western blot analysis of serum samples when tested with sialic-acid-specific <t>lectin,</t> SNA ( c ) Sugar analysis showing amount of Neu5Ac and Neu5Gc in serum samples. ( d ) Double immunostaining of Neu5Gc in mouse and ferret kidney tissue using chicken anti-Neu5Gc (Neu5Gc panels) and SubAB overlay followed by rabbit anti-SubA (SubAB panels). In a and b , F=ferret, H=human, B=bovine and M=mouse serum, respectively; + or − represent samples with or without neuraminidase treatment (see for full western blot images).
Lectin Sna Alkaline Phosphatase Conjugate La 6802 1, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lectin sna-alkaline phosphatase conjugate la-6802-1/product/EY Laboratories
Average 90 stars, based on 1 article reviews
lectin sna-alkaline phosphatase conjugate la-6802-1 - by Bioz Stars, 2026-02
90/100 stars
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fitc-conjugated maackia amurensis lectin mal-i  (AnaSpec)
90
AnaSpec fitc-conjugated maackia amurensis lectin mal-i
( a ) Western blot analysis showing the absence/presence of Neu5Gc in serum samples (10 μg) when tested with anti-Neu5Gc antibody. ( b ) Western blot analysis of serum samples when tested with sialic-acid-specific <t>lectin,</t> SNA ( c ) Sugar analysis showing amount of Neu5Ac and Neu5Gc in serum samples. ( d ) Double immunostaining of Neu5Gc in mouse and ferret kidney tissue using chicken anti-Neu5Gc (Neu5Gc panels) and SubAB overlay followed by rabbit anti-SubA (SubAB panels). In a and b , F=ferret, H=human, B=bovine and M=mouse serum, respectively; + or − represent samples with or without neuraminidase treatment (see for full western blot images).
Fitc Conjugated Maackia Amurensis Lectin Mal I, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc-conjugated maackia amurensis lectin mal-i/product/AnaSpec
Average 90 stars, based on 1 article reviews
fitc-conjugated maackia amurensis lectin mal-i - by Bioz Stars, 2026-02
90/100 stars
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agarose-conjugated sna-1 lectin  (EY Laboratories)
90
EY Laboratories agarose-conjugated sna-1 lectin
( a ) Western blot analysis showing the absence/presence of Neu5Gc in serum samples (10 μg) when tested with anti-Neu5Gc antibody. ( b ) Western blot analysis of serum samples when tested with sialic-acid-specific <t>lectin,</t> SNA ( c ) Sugar analysis showing amount of Neu5Ac and Neu5Gc in serum samples. ( d ) Double immunostaining of Neu5Gc in mouse and ferret kidney tissue using chicken anti-Neu5Gc (Neu5Gc panels) and SubAB overlay followed by rabbit anti-SubA (SubAB panels). In a and b , F=ferret, H=human, B=bovine and M=mouse serum, respectively; + or − represent samples with or without neuraminidase treatment (see for full western blot images).
Agarose Conjugated Sna 1 Lectin, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/agarose-conjugated sna-1 lectin/product/EY Laboratories
Average 90 stars, based on 1 article reviews
agarose-conjugated sna-1 lectin - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. ASGR1 was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.

Journal: Science Advances

Article Title: Targeted inhibition of hepatic de novo ceramide synthesis ameliorates MASH

doi: 10.1126/sciadv.adx2681

Figure Lengend Snippet: ( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. ASGR1 was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.

Article Snippet: Cells were stained with an ASGR1 antibody (Proteintech, catalog no. CL488-11739, Illinois, USA) for 30 min at 4°C in the dark.

Techniques: Imaging, Injection, Control, Labeling, Flow Cytometry, Marker, Western Blot, Expressing

( a ) Western blot analysis showing the absence/presence of Neu5Gc in serum samples (10 μg) when tested with anti-Neu5Gc antibody. ( b ) Western blot analysis of serum samples when tested with sialic-acid-specific lectin, SNA ( c ) Sugar analysis showing amount of Neu5Ac and Neu5Gc in serum samples. ( d ) Double immunostaining of Neu5Gc in mouse and ferret kidney tissue using chicken anti-Neu5Gc (Neu5Gc panels) and SubAB overlay followed by rabbit anti-SubA (SubAB panels). In a and b , F=ferret, H=human, B=bovine and M=mouse serum, respectively; + or − represent samples with or without neuraminidase treatment (see for full western blot images).

Journal: Nature Communications

Article Title: Ferrets exclusively synthesize Neu5Ac and express naturally humanized influenza A virus receptors

doi: 10.1038/ncomms6750

Figure Lengend Snippet: ( a ) Western blot analysis showing the absence/presence of Neu5Gc in serum samples (10 μg) when tested with anti-Neu5Gc antibody. ( b ) Western blot analysis of serum samples when tested with sialic-acid-specific lectin, SNA ( c ) Sugar analysis showing amount of Neu5Ac and Neu5Gc in serum samples. ( d ) Double immunostaining of Neu5Gc in mouse and ferret kidney tissue using chicken anti-Neu5Gc (Neu5Gc panels) and SubAB overlay followed by rabbit anti-SubA (SubAB panels). In a and b , F=ferret, H=human, B=bovine and M=mouse serum, respectively; + or − represent samples with or without neuraminidase treatment (see for full western blot images).

Article Snippet: For detection of sialic acid (Neu5Ac and Neu5Gc), lectin SNA-alkaline phosphatase conjugate (LA-6802-1, EY Laboratories) was used at 1/1,000 dilution in 1% bovine serum albumin (w/v) in PBS.

Techniques: Western Blot, Double Immunostaining